The paper summarizes yield trial results with soybeans over-expressing the TCS apyrase yield gene carried out over multiple years in the United States and Argentina. Soybean lines expressing psNTP9 showed significantly enhanced seed yields (up to more than 30%) when grown in multiple different field conditions at multiple field sites, including when the gene is introgressed into elite soybean germplasm. The transgenic lines have higher leaf chlorophyll and soluble protein contents and decreased stomatal density and cuticle permeability, traits that increase water use efficiency and likely contribute to the increased seed yields of field-grown plants. These altered properties are explained, in part, by genome-wide gene expressing changes induced by the transgene.